Anti-Complement Factor H Antibody ELISA 试剂盒
This 人 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 .
Quick Overview for Anti-Complement Factor H Antibody ELISA 试剂盒 (ABIN459353)
抗原
Anti-Complement Factor H Antibody
适用
人
检测方法
Colorimetric
实验类型
Indirect ELISA
应用范围
ELISA
样品类型
Serum, Plasma
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原理
- anti-complement factor H is an enzyme linked immunosorbent assay designed to detect IgG antibodies against complement factor H.
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品牌
- ELISA-VIDITEST
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Analytical Method
- Quantitative
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组件
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- ELISA strips coated with human factor H purified from human plasma STRIPS Ag 1 x 6 strips
- 50 μL Anti-CFH IgG standard STANDARD 1 vial
- 0.1 mL IgG-HRP conjugate - detection anti-human antibody labelled by horseradish peroxidase,101x concentrated CONJ 101x 1 vial
- 55 mL Wash buffer 10x concentrated WASH 10x 1 vial
- 60 mL Dilution buffer (DB) r.t.u. 1) DIL 1 vial
- 13 mL Chromogenic substrate (TMB-BF substrate) r.t.u. (TMB/H2O2) TMB-BF 1 vial
- 13 mL Stop solution r.t.u. (0.4 M sulfuric acid) STOP 1 vial
- Instruction manual
- Quality control certificate 1) r.t.u. ready to use
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试剂未包括
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- Distilled or deionised water for dilution of buffer and standard concentrates
- appropriate and calibrated equipment for pipetting
- temperature controlled incubator
- spectrophotometer or platereader with the appropriate filters
- empty microtiter plate for pre-incubation
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
- anti-complement factor H is an enzyme linked immunosorbent assay designed to detect IgG antibodies against complement factor H. The wells of the microtitrate plate are coated with purified human complement factor H. Antibodies against factor H present in serum sample bind to the immobilized factor H. Other antibodies, unbound to the factor H, are washed away during the next step. Then anti-human IgG antibodies labelled with horseradish peroxidase are added and those detect the antibodies from the sample that previously bound to factor H. The unbound labelled antibodies are washed away and the remaining labelled antibodies are visualized with a chromogenic substrate. The peroxidase activity leads to a change in colour of the solution. The reaction is stopped by adding an acidic solution. The colour intensity is directly proportional to the amount of anti-factor H antibodies in the sample.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- Anti-Complement Factor H Antibody
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物质类
- Antibody
抗原
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